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8349
NuRD Complex Antibody Sampler Kit

NuRD Complex Antibody Sampler Kit #8349

Western Blotting Image 1

Western blot analysis of extracts from HeLa and Jurkat cells using CHD3 Antibody.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using CHD4 (D8B12) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using MBD3 (N87) Antibody.

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Western Blotting Image 6

Western blot analysis of extracts from MCF7 and A-204 cells using MTA1 (D40D1) XP® Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using RBAP46 (V415) Antibody.

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Western Blotting Image 8

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IP Image 9

Immunoprecipitation of CHD4 from COS-7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CHD4 (D8B12) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using CHD4 (D8B12) Rabbit mAb.

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IF-IC Image 10

Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded mouse 4T1A mouse syngeneic tumor using MTA1 (D40D1) XP® Rabbit mAb.

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IF-IC Image 12

Confocal immunofluorescent analysis of HeLa cells using RBAP46 (V415) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MTA1 (D40D1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
CHD3 Antibody 4241 20 µl
  • WB
  • IP
H M Mk 260 Rabbit 
CHD4 (D8B12) Rabbit mAb 11912 20 µl
  • WB
  • IP
H M R Mk 260 Rabbit IgG
HDAC1 (10E2) Mouse mAb 5356 20 µl
  • WB
  • IP
H M R Mk 62 Mouse IgG1
HDAC2 (3F3) Mouse mAb 5113 20 µl
  • WB
  • IP
  • IF
H M R Mk 60 Mouse IgG1
MBD3 (N87) Antibody 14540 20 µl
  • WB
H M R Mk 32, 34 Rabbit 
MTA1 (D40D1) XP® Rabbit mAb 5647 20 µl
  • WB
  • IHC
H M R Mk 78-82 Rabbit IgG
RBAP46 (V415) Antibody 6882 20 µl
  • WB
  • IP
  • IF
H M R Mk 48 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

NuRD Complex Antibody Sampler Kit offers an economical means of detecting each target protein that composes the nucleosome remodeling and deacetylation complex (NuRD). The kit contains enough primary antibody to perform two western blot experiments with each primary antibody.

All antibodies contained in this kit detect endogenous levels of their respective target protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

The transcriptional repressor NuRD (nucleosome remodeling and histone deacetylase) complex is composed of multiple subunits, which include CHD3 (also known as Mi2-α), CHD4 (also known as Mi2-β), HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (1-6). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. HDAC1 and HDAC2 are highly homologous and are involved in histone deacetylation, chromatin remodeling and transcriptional repression, and are also found together in the mSin3A and MeCP1 complexes (7-9). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target the NuRD complex to its histone substrates (10). MBD3, a member of the methyl-CpG-binding domain protein family, and the metastasis associated genes MTA1, MTA2, and MTA3 were all found to be integral components of the NuRD complex (2,3,7,11).

  1. Tong, J.K. et al. (1998) Nature 395, 917-21.
  2. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
  3. Zhang, Y. et al. (1998) Cell 95, 279-89.
  4. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
  5. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
  6. Fujita, N. et al. (2003) Cell 113, 207-19.
  7. Ng, H.H. et al. (1999) Nat Genet 23, 58-61.
  8. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
  9. Zhang, Y. et al. (1997) Cell 89, 357-64.
  10. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
  11. Wade, P.A. et al. (1999) Nat Genet 23, 62-6.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Technical Support and Customer Service Closing Early Today

US Customer Service and Technical Support will be closing at 3:00 PM EST today. Any US orders placed today will ship on Tuesday, May 29.