| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| α-Amylase (D55H10) XP® Rabbit mAb | 3796 | 40 µl | 58 kDa | Rabbit IgG |
| CD31 (PECAM-1) (89C2) Mouse mAb | 3528 | 40 µl | 130 kDa | Mouse IgG1 |
| Keratin 17/19 (D32D9) XP® Rabbit mAb | 3984 | 40 µl | 48/41 kDa | Rabbit IgG |
| Pdx1 (D59H3) XP® Rabbit mAb | 5679 | 40 µl | 42 kDa | Rabbit IgG |
| PLA2G1B (D1T4C) Rabbit mAb | 14635 | 40 µl | 16 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
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Description
The Pancreatic Marker Antibody Sampler Kit provides an economical means to evaluate relevant pancreatic proteins. This kit contains enough primary antibody to perform four western blots per primary.
Storage
Background
The Pancreatic Marker Antibody Sampler Kit contains a variety of antibodies directed against established pancreatic proteins. α-Amylase catalyzes the cleavage of 1, 4-α-D-glucosidic bonds in oligosaccharides and polysaccharides (1). The enzyme is normally produced and secreted in salivary glands (salivary α-amylase or AMY1) and pancreas (pancreatic α-amylase or AMY2A) (1). CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells, and modulates cell adhesion, endothelial cell migration, and angiogenesis (2). Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (3,4). The pancreatic duodenal homeobox gene-1 (Pdx1) is a transcription factor that contributes to pancreas development, pancreatic β-cell differentiation, and mature β-cell function (5,6). It plays an essential role in the commitment of endoderm to a pancreatic and later β-cell phenotype (6,7). In the mature pancreas, Pdx1 expression is more restricted to the pancreatic β-cells (7), where it promotes the expression of genes important for β-cell functions, such as insulin, glucokinase, and Glut2 (8-10). Phospholipase A2 (PLA2) is a superfamily of enzymes that hydrolyze glycero-3-phosphocholines and release fatty acids and lysophospholipids (11). PLA2G1B is a member of this superfamily in the 1B group that is expressed most highly in the pancreatic acinar cells (12). Evidence suggests that PLA2G1B plays a role in the absorption and storage of extra energy as fats are metabolized (11,12).
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- Guz, Y. et al. (1995) Development 121, 11-8.
- Ohlsson, H. et al. (1993) EMBO J 12, 4251-9.
- Watada, H. et al. (1996) Diabetes 45, 1826-31.
- Waeber, G. et al. (1996) Mol Endocrinol 10, 1327-34.
- Labonté, E.D. et al. (2010) FASEB J 24, 2516-24.
- Cash, J.G. et al. (2011) Int J Obes (Lond) 35, 877-81.
Background References
Trademarks and Patents
Limited Uses
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