WB, IP
H
Endogenous
120-130
Rabbit IgG
#Q86W56
8505
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro179 of human PARG protein.
Background
Poly (ADP-ribose) glycohydrolase (PARG) is an enzyme that hydrolyzes poly (ADP-ribose) (PAR) formed by members of the PAR polymerase (PARP) enzyme family. Poly (ADP)-ribosylation is a post-translational modification that is catalyzed by PARP proteins. This modification involves polymerization of ADP-ribose from NAD+ to target proteins, such as histones and transcription factors, and plays a wide range of biological roles, including the response to DNA damage and transcriptional regulation (1,2). The mammalian PARG enzyme that catalyzes the removal of this modification exists as multiple isoforms. PARG isoforms 1-3 shuttle between the nucleus and cytoplasm and are responsible for most of the PARG activity. The smaller isoforms 4 and 5 reside in the cytoplasm (3-5). Research studies link altered PAR metabolism to inflammatory and autoimmune diseases, as well as neuronal degeneration (6-8). PARG inhibitors that increase PAR levels may sensitize cells to cancer treatments (e.g., cisplatin) and may help in the development of cancer therapies (9).
- Thomas, C. and Tulin, A.V. (2013) Mol Aspects Med 34, 1124-37.
- Li, N. and Chen, J. (2014) Mol Cells 37, 9-16.
- Meyer-Ficca, M.L. et al. (2004) Exp Cell Res 297, 521-32.
- Meyer-Ficca, M.L. et al. (2005) Int J Biochem Cell Biol 37, 920-6.
- Bonicalzi, M.E. et al. (2003) Biol Cell 95, 635-44.
- Cortes, U. et al. (2004) Mol Cell Biol 24, 7163-78.
- Masutani, M. et al. (2005) Cell Mol Life Sci 62, 769-83.
- Ying, W. and Swanson, R.A. (2000) Neuroreport 11, 1385-8.
- Fauzee, N.J. et al. (2010) Pathol Oncol Res 16, 469-78.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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