WB
H M R
Endogenous
62
Rabbit IgG
#P36871
5236
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly279 of human PGM1 protein.
Background
Phosphoglucomutase-1 (PGM1) catalyzes the interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P) in the breakdown and synthesis of glycogen. PGM1 expression is downregulated in hepatocellular carcinoma (HCC), suppressing the glycogen synthesis pathway and increasing glucose entry into glycolysis to support cancer proliferation (1). Studies show that TGF-β1 released by ovarian cancer cells induces p38 MAPK signaling in neighboring cancer-associated fibroblasts (CAFs), releasing cytokines and chemokines from CAFs. These cytokines and chemokines promote glycogen mobilization in ovarian cancer cells, stimulating cancer cell proliferation, invasion, and metastasis. Phosphorylation of Tyr353 on PGM1 is essential for its enzymatic activity and glycogen mobilization induced by CAFs (2).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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