WB
H
Endogenous
61
Rabbit
#Q9H8Y8
26003
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr264 of human GRASP55/GORASP2 protein. Antibodies are purified by peptide affinity chromatography.
Background
In unconventional protein secretion (UPS), cytoplasmic cargo proteins that do not have a signal peptide or a transmembrane domain are transported to the plasma membrane or extracellular matrix mainly to respond to cellular stresses (1,2). The Golgi reassembly-stacking protein 2 (GRASP55, also known as GORASP2) is essential to type III and type IV UPS (2,3). Studies show that mTORC1 phosphorylates GRASP55/GORASP2 and keeps it at the Golgi apparatus under non-stressed conditions. When cells are stressed, mTORC1 is inhibited, leading to dephosphorylation of GRASP55/GORASP2. Dephosphorylated GRASP55/GORASP2 relocates to autophagosomes and multivesicular bodies to promote UPS. In addition, Thr264 on GRASP55/GORASP2 was shown to seemingly play a key role in mTORC1-mediated phosphorylation of the protein (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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