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Phospho-GRASP55/GORASP2 (Thr264) Antibody #53726

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Inquiry Info. # 53726

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 61
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-GRASP55/GORASP2 (Thr264) Antibody recognizes endogenous levels of GRASP55/GORASP2 protein only when phosphorylated at Thr264. This antibody may detect a nonspecific band around 45 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr264 of human GRASP55/GORASP2 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    In unconventional protein secretion (UPS), cytoplasmic cargo proteins that do not have a signal peptide or a transmembrane domain are transported to the plasma membrane or extracellular matrix mainly to respond to cellular stresses (1,2). The Golgi reassembly-stacking protein 2 (GRASP55, also known as GORASP2) is essential to type III and type IV UPS (2,3). Studies show that mTORC1 phosphorylates GRASP55/GORASP2 and keeps it at the Golgi apparatus under non-stressed conditions. When cells are stressed, mTORC1 is inhibited, leading to dephosphorylation of GRASP55/GORASP2. Dephosphorylated GRASP55/GORASP2 relocates to autophagosomes and multivesicular bodies to promote UPS. In addition, Thr264 on GRASP55/GORASP2 was shown to seemingly play a key role in mTORC1-mediated phosphorylation of the protein (2).

    Alternate Names

    DKFZp434D156; FLJ13139; Golgi phosphoprotein 6; golgi reassembly stacking protein 2; golgi reassembly stacking protein 2, 55kDa; Golgi reassembly-stacking protein 2; Golgi reassembly-stacking protein of 55 kDa; GOLPH6; GORASP2; GORS2; GRASP55; GRS2; p59

    For Research Use Only. Not for Use in Diagnostic Procedures.
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