WB, IP
H
Endogenous
140
Rabbit
#Q9Y4G2
9842
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human PLEKHM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Pleckstrin homology domain-containing protein family member 1 (PLEKHM1) is a multivalent endolysosomal adaptor protein that contains an N-terminal RUN domain, two internal PH domains, and a C-terminal C1 domain/zinc-finger-like motif. Research studies have shown that PLEKHM1 plays a role in bone resorption by regulating the acidification of endosomal vesicles of osteoclasts. Loss-of-function mutations in PLEKHM1 promote osteoclast failure and impair bone resorption, which leads to osteopetrosis (1-3). Recent studies have shown that PLEKHM1 serves as a nexus for different trafficking pathways of the endolysosomal network by serving as the platform for the recruitment of Rab7, HOPS complex, and LC3/GABARAP family members. Indeed, loss of PLEKHM1 impairs the lysosomal degradation of cell-surface receptors and autophagic clearance of protein aggregates (4,5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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