WB
H M R
Endogenous
23
Rabbit
#P32119
7001
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PRDX2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Peroxiredoxin 2 (PRDX2, PRXII, NKEFB) is a ubiquitously expressed thioredoxin peroxidase. The enzyme catalyzes the reduction of hydrogen peroxide and organic hydroperoxides via the thioredoxin system (1). An antioxidant, PRDX2 neutralizes endogenous reactive oxygen species (ROS) and regulates cytokine-induced peroxide levels for normal cell function (2). Research studies have shown that PRDX2 plays important roles in inflammation, cancer, and natural killer (NK) cell activation (3). During cancer progression, PRDX2 is upregulated and protects cancer cells from oxidative stress-induced apoptosis (4, 5). In inflammatory diseases such as infection, myocardial infarction, and ischemia, PRDX2 not only protects (host) cells from oxidative stress-induced death, but is also released into extracellular space to trigger local inflammation and to activate NK cells for innate immune response (6, 7).
- Peskin, A.V. et al. (2007) J Biol Chem 282, 11885-92.
- Poole, L.B. et al. (2011) Curr Protoc Toxicol Chapter 7, Unit7.9.
- Ishii, T. et al. (2012) J Clin Biochem Nutr 50, 91-105.
- Stresing, V. et al. (2013) Oncogene 32, 724-35.
- Lu, W. et al. (2014) Mol Cell Biochem 387, 261-70.
- Knoops, B. et al. (2016) Mol Cells 39, 60-4.
- Jin, X. et al. (2017) Sci Rep 7, 6970.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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