WB, IP
H M
Endogenous
98
Rabbit IgG
#Q9Y2R2
26191
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro451 of human PTPN22 protein.
Background
PTPN22 (Lyp/PEP) is a cytoplasmic phosphatase expressed by hematopoietic cells (1,2). PTPN22 associates with the tyrosine kinase Csk to inhibit T cell receptor signaling through inactivation of Src kinases (3,4). Csk phosphorylates Src kinases on an inhibitory tyrosine, while PTPN22 dephosphorylates an activating site (4). PTPN22(-/-) mice have higher levels of activated Lck than wild-type, resulting in greater T cell expansion and increased serum antibody levels (5). Research studies have shown that a single-nucleotide polymorphism, 1858T of the PTPN22 gene which encodes the amino acid substitution R620W, confers increased risk for multiple autoimmune diseases including type I diabetes, rheumatoid arthritis, systemic lupus erythematosus, and Graves disease (6-9). Interestingly, although the R620W substitution disrupts the interaction between Csk and PTPN22, it is actually a gain-of-function mutation resulting in increased phosphatase activity (6,10,11). Recent evidence suggests that the autoimmune phenotype associated with the R620W variant is the result of increased calpain-mediated degradation and decreased protein levels of PTPN22 (12).
- Cohen, S. et al. (1999) Blood 93, 2013-24.
- Matthews, R.J. et al. (1992) Mol Cell Biol 12, 2396-405.
- Cloutier, J.F. and Veillette, A. (1996) EMBO J 15, 4909-18.
- Cloutier, J.F. and Veillette, A. (1999) J Exp Med 189, 111-21.
- Hasegawa, K. et al. (2004) Science 303, 685-9.
- Bottini, N. et al. (2004) Nat Genet 36, 337-8.
- Begovich, A.B. et al. (2004) Am J Hum Genet 75, 330-7.
- Kyogoku, C. et al. (2004) Am J Hum Genet 75, 504-7.
- Velaga, M.R. et al. (2004) J Clin Endocrinol Metab 89, 5862-5.
- Vang, T. et al. (2005) Nat Genet 37, 1317-9.
- Rieck, M. et al. (2007) J Immunol 179, 4704-10.
- Zhang, J. et al. (2011) Nat Genet 43, 902-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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