WB, IP, ChIP
H
Endogenous
45
Rabbit IgG
#O75469
8856
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human PXR protein.
Background
The pregnane X receptor (PXR) is part of the nuclear hormone receptor superfamily and is a master regulator of xenobiotic metabolism (1). PXR was initially described as being activated by pregnanes and inducing expression of the CYP3A family of steroid hydroxylases (2,3). Similar to PPAR and CAR nuclear receptors, PXR utilizes RXR as a heterodimeric partner and controls target hepatic enzyme genes to control liver metabolism in response to a wide variety of drugs (4). However, PXR is strikingly evolutionarily divergent, and the ligand-binding domain only shares around 75% identity across species. Compounds that readily activate human PXR, such as rifampicin, have little activity in rodents (5,6). In humans, in addition to naturally occurring steroids, PXR is activated by numerous xenobiotics, including anti-cancer drugs paclitaxel and tamoxifen, the HIV protease inhibitor ritonavir, and the anti-diabetic troglitazone (5,7-9). PXR has been implicated in numerous conditions, including inflammatory bowel disease, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD) (10-12). PXR plays a protective role in many tumor types and its expression is generally correlated with better prognosis (13-15).
- Kliewer, S.A. et al. (2002) Endocr Rev 23, 687-702.
- Kliewer, S.A. et al. (1998) Cell 92, 73-82.
- Lehmann, J.M. et al. (1998) J Clin Invest 102, 1016-23.
- Waxman, D.J. (1999) Arch Biochem Biophys 369, 11-23.
- Jones, S.A. et al. (2000) Mol Endocrinol 14, 27-39.
- LeCluyse, E.L. (2001) Chem Biol Interact 134, 283-9.
- Dussault, I. et al. (2001) J Biol Chem 276, 33309-12.
- Synold, T.W. et al. (2001) Nat Med 7, 584-90.
- Desai, P.B. et al. (2002) Drug Metab Dispos 30, 608-12.
- Langmann, T. et al. (2004) Gastroenterology 127, 26-40.
- Makishima, M. (2005) J Pharmacol Sci 97, 177-83.
- Lee, J.H. et al. (2008) Mol Pharm 5, 60-6.
- Ouyang, N. et al. (2010) Br J Cancer 102, 1753-61.
- Masuyama, H. et al. (2016) Int J Oncol 49, 1211-20.
- Fujimura, T. et al. (2012) Cancer Sci 103, 176-80.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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