WB, IP
H
Endogenous
119
Rabbit IgG
#P15918
5896
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human RAG1 protein.
Background
The sequences encoding antigen receptors are split into multiple germline segments which are then combined by a process called V(D)J recombination during immune cells development. A variable (V) segment is combined with a joining (J) segment, and in some cases a D (Diversity) segment, to create the antigen-binding portion of the receptor. The recombined V(D)J segment is then spliced into exons that encode the constant region to produce mature mRNA (1,2). This essential process required for the development of functional immune T and B cells creates a vast diversity in these receptors (3,4). Initiation of this process follows binding of RAG1 (recombination activating gene 1) and RAG2 to the conserved recombination signal sequences (RSS) and the introduction of a double-strand break between the RSS and the coding sequence (5,6). RAG1 and RAG2 genes are located immediately adjacent to each other in the genome and lack introns in their coding regions in many species. RAG1 and RAG2 are coexpressed only in the B and T cell lineages and both are required for cleavage activity (7). RAG1 and RAG2 can also function as transposases, contributing to chromosomal translocations and lymphoid malignancy (8,9). Mutations in the RAG genes are associated with a spectrum of combined immune deficiencies in humans (10,11).
- Schatz, D.G. (2004) Semin Immunol 16, 245-56.
- Gellert, M. (2002) Annu Rev Biochem 71, 101-32.
- Alt, F.W. et al. (1992) Ann N Y Acad Sci 651, 277-94.
- Sadofsky, M.J. (2001) Nucleic Acids Res 29, 1399-409.
- Swanson, P.C. (2004) Immunol Rev 200, 90-114.
- Swanson, P.C. et al. (2009) Adv Exp Med Biol 650, 1-15.
- Fugmann, S.D. et al. (2000) Annu Rev Immunol 18, 495-527.
- Hiom, K. et al. (1998) Cell 94, 463-70.
- Agrawal, A. et al. (1998) Nature 394, 744-51.
- Villa, A. et al. (1999) J Clin Immunol 19, 87-97.
- Corneo, B. et al. (2000) J Biol Chem 275, 12672-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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