Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
Phospho-Rb (Ser780) (D59B7) Rabbit mAb | 8180 | 20 µl | 110 kDa | Rabbit IgG |
Phospho-Rb (Ser795) Antibody | 9301 | 20 µl | 110 kDa | Rabbit  |
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb | 8516 | 20 µl | 110 kDa | Rabbit IgG |
Rb (4H1) Mouse mAb | 9309 | 20 µl | 110 kDa | Mouse IgG2a |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat  | |
Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse  |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Rb Antibody Sampler Kit provides reagents and protocols to investigate cell cycle progression within cells. The kit contains primary and secondary antibodies to perform two Western blot experiments with each antibody.
Storage
Background
The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
- Sherr, C.J. (1996) Science 274, 1672-7.
- Nevins, J.R. (1992) Science 258, 424-9.
- Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90.
- Hu, Q.J. et al. (1990) EMBO J 9, 1147-55.
- Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83.
- Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
- Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301.
- Kitagawa, M. et al. (1996) EMBO J 15, 7060-9.
- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
Background References
Trademarks and Patents
Limited Uses
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