WB, IP
H Mk
Endogenous
110, 135
Rabbit IgG
#P98175
8241
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly53 of human RBM10 protein.
Background
RBM10 is an RNA binding protein and a paralog to tumor suppressor RBM5 (1,2). Alternative splicing creates two highly expressed variants that differ by one exon. RBM10 controls alternative splicing and exon skipping of mRNAs, including Fas and Bcl-x (3,4). RBM10 has been shown to affect apoptosis via up regulation of TNF-α mRNA (5). In lung cancer, RBM10 mutations disrupt the splicing of NUMB, a regulator of Notch signaling (6,7). Fusion of the RBM10 gene with TFE3 has been found in renal cell carcinoma (8-9). Mutations of RBM10 causes TARP syndrome, an x-linked, lethal disorder characterized by various developmental defects (10-12).
- Timmer, T. et al. (1999) Genomics 60, 238-40.
- Bonnal, S. et al. (2008) Mol Cell 32, 81-95.
- Wang, Y. et al. (2013) EMBO Mol Med 5, 1431-42.
- Inoue, A. et al. (2014) FEBS Lett 588, 942-7.
- Wang, K. et al. (2012) J Cell Death 5, 1-19.
- Bechara, E.G. et al. (2013) Mol Cell 52, 720-33.
- Hernández, J. et al. (2016) RNA Biol 13, 466-72.
- Just, P.A. et al. (2016) Genes Chromosomes Cancer 55, 541-8.
- Argani, P. et al. (2017) Am J Surg Pathol 41, 655-62.
- Gorlin, R.J. et al. (1970) Am J Dis Child 119, 176-8.
- Johnston, J.J. et al. (2010) Am J Hum Genet 86, 743-8.
- Gripp, K.W. et al. (2011) Am J Med Genet A 155A, 2516-20.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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