Revision 1

#9968

Store at -20C

Rho-GTPase Antibody Sampler Kit

1 Kit

(6 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
Cdc42 (11A11) Rabbit mAb246620 µl21 kDaRabbit
Phospho-Rac1/cdc42 (Ser71) Antibody246120 µl28 kDaRabbit
RhoA (67B9) Rabbit mAb211720 µl21 kDaRabbit IgG
RhoB Antibody209820 µl21 kDaRabbit
RhoC (D40E4) Rabbit mAb343020 µl21 kDaRabbit IgG
Rac1/2/3 Antibody246520 µl21 kDaRabbit
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Rho-GTPase Antibody Sampler Kit contains reagents to examine aspects of cell migration, adhesion, proliferation and differentiation in cells. This kit includes enough primary and secondary antibodies to perform two Western blot experiments per each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation (1). RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity (2-5). Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development (6). Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding (7). Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases (8). Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain (8-10). Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization (11).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from mouse and rat brain tissue using RhoB Antibody.
undefined Image 1: Rho-GTPase Antibody Sampler Kit
Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.
undefined Image 2: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using RhoA (67B9) Rabbit mAb.
undefined Image 3: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of A431 cell extracts, untreated (-) or EGF treated (+), using Phospho-Rac1/cdc42 (Ser71) Antibody (upper) or Rac1/Cdc42 Antibody #4651 (lower).
undefined Image 4: Rho-GTPase Antibody Sampler Kit
Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody.
undefined Image 5: Rho-GTPase Antibody Sampler Kit
Western blot analysis of extracts from various cell lysates, using Cdc42 (11A11) Rabbit mAb.
undefined Image 6: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell types using RhoC (D40E4) Rabbit mAb.
undefined Image 7: Rho-GTPase Antibody Sampler Kit
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
undefined Image 8: Rho-GTPase Antibody Sampler Kit
Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoB Antibody.
undefined Image 9: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb.
undefined Image 10: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded human thymoma using RhoA (67B9) Rabbit mAb.
undefined Image 11: Rho-GTPase Antibody Sampler Kit
Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoC (D40E4) Rabbit mAb.
undefined Image 12: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human spleen using RhoA (67B9) Rabbit mAb.
undefined Image 13: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded normal human lymph node using RhoA (67B9) Rabbit mAb.
undefined Image 14: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using RhoA (67B9) Rabbit mAb.
undefined Image 15: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using RhoA (67B9) Rabbit mAb.
undefined Image 16: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse spleen using RhoA (67B9) Rabbit mAb.
undefined Image 17: Rho-GTPase Antibody Sampler Kit
Immunohistochemical analysis of paraffin-embedded mouse prostate using RhoA (67B9) Rabbit mAb.
undefined Image 18: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma (left) or human prostate adenocarcinoma (right) using RhoA (67B9) Rabbit mAb (top) or a RhoA Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human RhoA. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
undefined Image 19: Rho-GTPase Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded normal human liver using RhoA (67B9) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

undefined Image 20: Rho-GTPase Antibody Sampler Kit

Immunohistochemical analysis of paraffin-embedded 239T cell pellet, untransfected (left) or RhoA-transfected (right), using RhoA (67B9) Rabbit mAb. Note endogenous expression in 293T cells.

undefined Image 21: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Simple WesternTM analysis of lysates (1 mg/ml) from HeLa cells using RhoA (67B9) Rabbit mAb #2117. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 2-40 kDa.
undefined Image 22: Rho-GTPase Antibody Sampler Kit
Confocal immunofluorescent analysis of control 293T cells (left, positive) or CRISPR/Cas9 RhoA knockout (KO) 293T cells (right, negative) using RhoA (67B9) Rabbit mAb #2117 (green) and DAPI #4083 (blue).
undefined Image 23: Rho-GTPase Antibody Sampler Kit
Western blot analysis of extracts from control 293T cells (lane 1) or CRISPR/Cas9 RhoA knockout (KO) 293T cells (lane 2) using RhoA (67B9) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
undefined Image 24: Rho-GTPase Antibody Sampler Kit
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.