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9968
Rho-GTPase Antibody Sampler Kit

Rho-GTPase Antibody Sampler Kit #9968

Western Blotting Image 1

Western blot analysis of extracts from various cell lysates, using Cdc42 (11A11) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of A431 cell extracts, untreated (-) or EGF treated (+), using Phospho-Rac1/cdc42 (Ser71) Antibody (upper) or Rac1/Cdc42 Antibody #4651 (lower).

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines, using RhoA (67B9) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from mouse and rat brain tissue using RhoB Antibody.

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Western Blotting Image 5

Western blot analysis of extracts from various cell types using RhoC (D40E4) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from COS, C6, NIH/3T3 and HEK293 cells, using Rac1/2/3 Antibody.

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 8

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoA (67B9) Rabbit mAb.

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Western Blotting Image 9

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoB Antibody.

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Western Blotting Image 10

Western blot analysis of recombinant GST-tagged RhoA, RhoB and RhoC using RhoC (D40E4) Rabbit mAb.

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Flow Cytometry Image 11

Flow cytometric analysis of Jurkat cells, using Rac1/2/3 Antibody (blue) compared to a nonspecific negative control antibody (red).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cdc42 (11A11) Rabbit mAb 2466 20 µl
  • WB
H M R B 21 Rabbit 
Phospho-Rac1/cdc42 (Ser71) Antibody 2461 20 µl
  • WB
H 28 Rabbit 
RhoA (67B9) Rabbit mAb 2117 20 µl
  • WB
H M R Mk B 21 Rabbit IgG
RhoB Antibody 2098 20 µl
  • WB
H M R Mk 21 Rabbit 
RhoC (D40E4) Rabbit mAb 3430 20 µl
  • WB
H M Mk 21 Rabbit IgG
Rac1/2/3 Antibody 2465 20 µl
  • WB
  • F
H M R Mk B 21 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Rho-GTPase Antibody Sampler Kit contains reagents to examine aspects of cell migration, adhesion, proliferation and differentiation in cells. This kit includes enough primary and secondary antibodies to perform two Western blot experiments per each primary antibody.

Cdc42 (11A11) Rabbit mAb detects endogenous levels of total Cdc42 protein and does not cross-react with other small GTPases. Phospho-Rac1/cdc42 (Ser71) Antibody detects endogenous Rac1/cdc42 only when phosphorylated at Ser71 and may also recognize phospho-RhoA (Ser73). Rac 1/2/3 Antibody detects endogenous levels of total Rac1/2/3 proteins. RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. RhoB Antibody recongnizes endogenous levels of RhoB. RhoC (D40E4) XP® Rabbit mAb recongnizes endogenous levels of RhoC.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr138 of human Rac1, corresponding to residues surrounding Ser71 of human Rac1/cdc42, and corresponding to residues near the carboxy terminus of RhoB . Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Lys135 of human Cdc42, residues near the carboxy terminus of human RhoA, and to residues corresponding to the carboxy terminus of human RhoC.

The Rho family of small GTPases, including Rho, Rac, and cdc42, act as molecular switches to regulate processes such as cell migration, adhesion, proliferation and differentiation (1). RhoA, RhoB and RhoC are all highly homologous but appear to have divergent biological functions. The best characterized of these proteins, RhoA, regulates acomysin contractility, cytokinesis, focal adhesion assembly and cell polarity (2-5). Mammalian Rac exists as three isoforms (Rac1, Rac2 and Rac3) that show high sequence similarity. Well-characterized Rac1 and cdc42 are ubiquitously expressed and play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, and cell growth and development (6). Phosphorylation of Rac1 at a putative Akt site (Ser71) may limit Rac1 activity through inhibition of GTP binding (7). Rac2 is expressed in cells of hematopoietic origin, while Rac3 is highly expressed in brain and in many other tissues. The Vav family of guanine-nucleotide exchange factors mediates activation of Rho/Rac family small GTPases (8). Negative regulation of Rho-activity members of the p190 RhoGAP family (p190-A and p190-B) may be controlled by Src phosphorylation of Tyr residues, activating the p190 GAP domain (8-10). Furthermore, Rho GDP dissociation inhibitor (RhoGDI) associates with Rho/Rac to negatively regulate nucleotide exchange membrane localization (11).

  1. Kwon T et al. (2000) J Biol Chem 275, 423–8
  2. Bi, D. et al. (2005) Circ Res 96, 890-7.
  3. Dovas, A. and Couchman, J.R. (2005) Biochem. J. 390, 1-9.
  4. Kimura, K. et al. (2000) J Biol Chem 275, 17233-6.
  5. Barry, S.T. and Critchley, D.R. (1994) J Cell Sci 107 ( Pt 7), 2033-45.
  6. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
  7. Van Keymeulen, A. et al. (2006) J Cell Biol 174, 437-45.
  8. Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
  9. Sordella, R. et al. (2003) Cell 113, 147-58.
  10. Chang, J.H. et al. (1995) J Cell Biol 130, 355-68.
  11. Roof, R.W. et al. (1998) Mol Cell Biol 18, 7052-63.
Entrez-Gene Id
998 , 5879 , 5880 , 5881 , 387 , 388 , 389
Swiss-Prot Acc.
P60953 , P63000 , P15153 , P60763 , P61586 , P62745 , P08134
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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Product # Size Price
9968T
1 Kit (6 x 20 µl) $ 423.0