WB
H M R Mk
Endogenous
65, 75, 100
Rabbit
#P23246
6421
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SFPQ protein. Antibodies are purified by peptide affinity chromatography.
Background
Splicing factor proline-glutamine rich (SFPQ), also commonly referred to as PTB-associated splicing factor (PSF), is an essential and ubiquitous nucleic acid-binding protein that is involved in a wide range of cellular processes. SFPQ belongs to the Drosophila melanogaster behavior, human splicing (DBHS) protein family along with paraspeckle protein 1 (PSPC1) and non-POU domain-containing octamer-binding protein (NONO) (1). DBHS proteins are a unique family of RNA-binding proteins that recruit various proteins and nucleic acids involved in transcriptional activation and repression, as well as transcript localization and splicing, and DNA damage repair (2). SFPQ and NONO are also core components of paraspeckles, which are subnuclear bodies distinct from nuclear speckles that are defined by the colocalization of SFPQ, NONO, and other proteins with the long noncoding RNA NEAT1 (2). The biological function of paraspeckles is currently unknown, but they are believed to be important for gene regulation in the presence of cellular stressors (3). Although SFPQ is typically localized to the nucleus, multiple studies indicate that cytoplasmic SFPQ levels are important for proper motor neuron differentiation and maintenance (4,5). SFPQ has also been linked to multiple neurological disorders, including Alzheimer’s disease (AD) and amyotrophic lateral sclerosis (ALS) (6-9).
- Bond, C.S. and Fox, A.H. (2009) J Cell Biol 186, 637-44.
- Knott, G.J. et al. (2016) Nucleic Acids Res 44, 3989-4004.
- Lim, Y.W. et al. (2020) Int J Mol Sci 21, 7151.
- Thomas-Jinu, S. et al. (2017) Neuron 94, 322-336.e5.
- Kanai, Y. et al. (2004) Neuron 43, 513-25.
- Younas, N. et al. (2020) Acta Neuropathol 140, 317-339.
- Lu, J. et al. (2018) J Alzheimers Dis 61, 1311-1321.
- Ke, Y.D. et al. (2012) PLoS One 7, e35678.
- Luisier, R. et al. (2018) Nat Commun 9, 2010.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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