WB
H
Endogenous
75
Rabbit IgG
#Q6IA17
59307
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SIGIRR protein.
Background
SIGIRR (Single Ig IL-1-related receptor), also known as IL-1R8, belongs to the IL-1 receptor family characterized by the presence of a common intracellular Toll-IL-1 resistance (TIR) domain and extracellular Ig-like domains (1-3). SIGIRR is widely expressed in several epithelial tissues such as liver, lung, and kidney, and in leukocytes including monocytes, T and B lymphocytes, dendritic cells, and natural killer (NK) cells. It negatively modulates the activation upon ligand binding of certain TIR-containing receptors including members of IL-1 receptor family and TLR family (4-6). Recently SIGIRR was identified as a crucial checkpoint receptor in NK cells and a potential therapeutic target in several pathological conditions (7,8).
- Garlanda, C. et al. (2013) Immunity 39, 1003-18.
- Kang, J.Y. and Lee, J.O. (2011) Annu Rev Biochem 80, 917-41.
- O'Neill, L.A. (2008) Immunol Rev 226, 10-8.
- Wald, D. et al. (2003) Nat Immunol 4, 920-7.
- Anselmo, A. et al. (2016) Cardiovasc Res 111, 373-84.
- Garlanda, C. et al. (2004) Proc Natl Acad Sci U S A 101, 3522-6.
- Molgora, M. et al. (2017) Nature 551, 110-114.
- Molgora, M. et al. (2018) Scand J Immunol 88, e12705.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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