WB, IHC-P
H M R
Endogenous
40
Rabbit IgG
#Q9UJZ1
30968
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val182 of human SLP-2 protein.
Background
Stomatin-like protein 2 (SLP-2 and also known as STOML2) is a lipid-anchored mitochondrial protein that is part of a large protein complex that regulates mitochondrial biogenesis and function. Proteomic studies identified SLP-2 as a widely expressed mitochondria-enriched protein (1). As a member of both the stomatin family and stomatin-prohibitin-flotillin-HfLC/K (SPFH) superfamily of proteins, SLP-2 forms large hetero-oligomeric complexes with other mitochondrial proteins, including prohibtin, mitofusin 2, and cardiolipin (2, 3). SLP-2 contains a highly conserved SPFH domain that mediates its ability to associate with the mitochondrial inner membrane and form specialized membrane microdomains. As an inner membrane organizer of other mitochondrial proteins, SLP-2 performs multiple mitochondrial functions, including regulation of mitochondrial biogenesis, energy/calcium homeostasis, translation, and mitochondrial-mediated cellular stress responses (3, 4, 5, 6, 7, 8). Enhanced SLP-2 expression is also associated with several human cancers, including gallbladder, rectal, and gastric cancer (9, 10, 11).
- Da Cruz, S. et al. (2003) J Biol Chem 278, 41566-71.
- Hájek, P. et al. (2007) J Biol Chem 282, 5670-81.
- Christie, D.A. et al. (2011) Mol Cell Biol 31, 3845-56.
- Da Cruz, S. et al. (2010) Cell Calcium 47, 11-8.
- Wang, Y. et al. (2009) Cancer Biol Ther 8, 1651-8.
- Christie, D.A. et al. (2012) J Immunol 189, 4349-60.
- Tondera, D. et al. (2009) EMBO J 28, 1589-600.
- Mitsopoulos, P. et al. (2017) PLoS One 12, e0179967.
- Wang, W.X. et al. (2014) Tumour Biol 35, 419-23.
- Liu, Z. et al. (2014) Oncol Rep 31, 153-60.
- Bartolome, A. et al. (2016) APMIS 124, 271-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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