WB, IP
H M R
Endogenous
150
Rabbit
#O60641
9892
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp487 of human SNAP-91/AP180 protein. Antibodies are purified by peptide affinity chromatography.
Background
Clathrin-coated assembly protein SNAP-91, also known as AP180, is an adaptor protein that regulates the endocytosis of proteins and receptors in the membranes at synaptic vesicles (VS), and in the sorting and clustering synaptic vesicle cargo (1,2). SNAP-91/AP180 regulates the formation of clathrin lattices on lipid templates in vitro in conjunction with clathrin assembly lymphoid myeloid leukemia (CALM), and often interacts with other endocytic pathways, including the adaptor protein-complex AP-2 (3,4). SNAP-91/AP180 modulates the invagination of the clathrin-coated pit (CCP) by facilitating the clathrin assembly (5). Mutations in the C. elegans SNAP-91/AP180 neuronal homologue unc11 induces large endocytic vesicles and defective neurotransmitter processes (5,6). SNAP-91/AP180 has been associated with and is related to schizophrenia risk variants, resulting in deficits at the pre- and post-synaptic neuronal function (7). In the tau transgenic mouse model of Alzheimer's disease, Tg2576, or the overexpression of SNAP-91/AP180, a selective clathrin-dependent endocytosis of monomeric Aβ peptides results in a greater uptake of Aβ 1-42 compared to Aβ 1-40, increasing the toxicity of cells due to Aβ peptide imbalance (8,9). SNAP-91/AP180 has been proposed as a candidate biomarker for glioblastoma (GBM), along with COL3A1, where a reduction of levels of these proteins was found in patients with GBM and other malignant gliomas (10).
- Schrode, N. et al. (2019) Nat Gen 1475-85.
- Gordon, S.L. and Cousin, M.A. (2016) Front Synaptic Neurosci 8, 1.
- Nonet, M.L. et al. (1999) Mol Biol Cell 10, 2343-60.
- Dittman, J. and Ryan, T.A. (2009) Annu Rev Cell Dev Biol 25, 133-60.
- von Kleist, L. et al. (2011) Cell 146, 471-84.
- Ford, M.G. et al. (2001) Science 291, 1051-5.
- Maritzen, T. et al. (2012) Biol Cell 104, 588-602.
- Wesén, E. et al. (2017) Sci Rep 7, 2021.
- Jin, S. et al. (2016) J Biol Chem 291, 19590-606.
- Gao, Y.F. et al. (2016) Oncotarget 7, 70494-503.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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