WB, IP, FC-FP
H
Endogenous
70
Rabbit IgG
#P35716
6664
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro323 of human SOX11 protein.
Background
SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins typically do not mediate transcription directly, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1). SOX11 is generally described as a neural transcription factor, whose expression is critical for the establishment of neuronal identity in the embryonic mammalian brain (2). It has furthermore been reported to function as a pioneer transcription factor, able to bind nucleosomes and influence the transcription of genes in regions of closed chromatin, in similar fashion to its close relative SOX2 (3). Postnatal expression of SOX11 is reported to be uncommon, but aberrant SOX11 expression has been reported in numerous malignancies, including mantle cell lymphoma, Burkitt's lymphoma, and various lymphoblastic leukemias (4-6).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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