WB
H Mk
Endogenous
17
Rabbit IgG
#Q04837
6742
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of human SSBP1 protein.
Background
The mitochondrial DNA replisome requires the activity of single-stranded DNA-binding protein (SSBP1, mtSSB), DNA Polymerase γ (POLG), and the mitochondrial DNA helicase (twinkle/PEO1) (1). SSBP1 enhances the functions of both POLG and PEO1 by binding to and stabilizing single-stranded DNA at the replication fork; knockdown of SSBP1 in vitro causes a gradual depletion of mitochondrial DNA and an acute depletion of 7S DNA (2). Mutations in SSBP1 underlie a variety of disorders, including optic atrophy, nephropathy (3), Pearson syndrome, Leigh syndrome, and Kearns-Sayre syndrome (4-6). SSBP1 has emerged as a potential therapeutic target for glioblastoma (7) and non-small cell lung cancer (NSCLC) (8), as controlled depletion of SSBP1 increases mitochondrial reactive oxygen species (ROS) and sensitizes these cancer cells to temozolomide and radiation therapy, respectively (7,8). The increase in mitochondrial ROS observed with SSBP1 depletion is linked to a corresponding decrease in nuclear ROS (9). ROS-sensing kinase Chk1 phosphorylates SSBP1 at Ser67, causing SSBP1 sequestration in the cytoplasm and limiting the translation of mitochondrial genes (9). This pathway may confer resistance to platinum-based therapies in ovarian cancers (9).
- Korhonen, J.A. et al. (2004) EMBO J 23, 2423-9.
- Ruhanen, H. et al. (2010) Biochim Biophys Acta 1803, 931-9.
- Del Dotto, V. et al. (2020) J Clin Invest 130, 108-125.
- Gustafson, M.A. et al. (2019) PLoS One 14, e0221829.
- Gustafson, M.A. et al. (2021) DNA Repair (Amst) 107, 103212.
- Lee, Y. et al. (2021) Genes (Basel) 12, 284. doi: 10.3390/genes12020284.
- Su, J. et al. (2022) J Transl Med 20, 440.
- Wang, Y. et al. (2017) J Cancer 8, 1400-1409.
- Zhang, J. et al. (2023) Cell 186, 2361-2379.e25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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