WB, IP, eCLIP
H M R Mk
Endogenous
27
Rabbit IgG
#Q9NP77
29101
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Hamster, Chicken, Bovine, Dog, Pig, Guinea Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro93 of human SSU72 protein.
Background
SSU72 is a protein phosphatase originally identified through its interaction with TFIIB as a factor required for transcription and RNA processing (1,2). While SSU72 has been known as a phosphatase of the RNA polymerase II carboxy-terminal domain at Ser5 of the heptapeptide repeat, additional studies suggest a role for SSU72 as a phosphatase for Ser7 of the heptapeptide repeat as well (3-9). The activities of SSU72 are thought to play a critical role in coupling transcription with pre-mRNA 3’ end processing (10-12). SSU72 is required for gene looping that connects the gene promoter with the terminator region, leading to proper initiation of transcription and enforcement of transcriptional directionality on promoters that may other wise act as bidirectional promoters (13-14). In addition to a role in transcription, SSU72 acts as a cohesin binding protein that regulates cohesion between sister chromatid arms during mitosis or meiosis (15).
- Sun, Z.W. and Hampsey, M. (1996) Mol Cell Biol 16, 1557-66.
- Pappas, D.L. and Hampsey, M. (2000) Mol Cell Biol 20, 8343-51.
- Ganem, C. et al. (2003) EMBO J 22, 1588-98.
- Krishnamurthy, S. et al. (2004) Mol Cell 14, 387-94.
- Hausmann, S. et al. (2005) J Biol Chem 280, 37681-8.
- Reyes-Reyes, M. and Hampsey, M. (2007) Mol Cell Biol 27, 926-36.
- Werner-Allen, J.W. et al. (2011) J Biol Chem 286, 5717-26.
- Zhang, D.W. et al. (2012) J Biol Chem 287, 8541-51.
- Bataille, A.R. et al. (2012) Mol Cell 45, 158-70.
- He, X. et al. (2003) Genes Dev 17, 1030-42.
- Steinmetz, E.J. and Brow, D.A. (2003) Mol Cell Biol 23, 6339-49.
- Xiang, K. et al. (2010) Nature 467, 729-33.
- Wang, Y. et al. (2010) Curr Biol 20, 548-53.
- Tan-Wong, S.M. et al. (2012) Science 338, 671-5.
- Kim, H.S. et al. (2010) EMBO J 29, 3544-57.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation eCLIP: eCLIP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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