WB, IP
H M R Mk
Endogenous
70
Rabbit
#Q92783
8027
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Dog, Pig, Horse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp286 of human STAM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Signal Transducing Adaptor Molecule 1 (STAM1) is a ubiquitously expressed adaptor protein containing an SH3 domain and an ITAM motif. Initial research studies demonstrated that STAM1 undergoes tyrosine phosphorylation following treatment with numerous cytokines and growth factors (1). Subsequent research studies identified STAM1 as a component of the ESCRT-0 complex, which mediates the endocytic sorting of ubiquitinated membrane proteins to the lysosomal compartment for degradation (2). STAM1 harbors a tandemly-oriented VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting motif) that facilitates STAM1 binding to ubiquitinated cargo proteins within the endosomal compartment (3,4). Gene targeting studies have revealed that STAM1 and STAM2 cooperate to promote thymic T-cell development and survival (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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