WB, IF-F, IF-IC
H M R
Endogenous
26
Rabbit IgG
#O43761
9143
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:400 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #29745.
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SYNGR3 protein.
Background
Synaptogyrin, or SYNGR, are a family of tyrosine-phosphorylated proteins, including neuronal SYNGR1 and SYNGR3 that are found in synaptic vesicles and contribute to the proper synapse function. Synaptogyrin-2 (SYNGR2) expresses ubiquitously and it is not only associated with synaptic vesicles, but also plays an important role in exocytosis processes (1,3). In addition, it has been shown that SYNGRs modulate calcium currents in excitable cells during potassium chloride-dependent exocytosis (3). SYNGR3 and SYNGR1 specifically localize in synaptic vesicles. SYNGR1 modulates synaptic vesicle function similar to SYNGR3 (2,3). SYNGR1 and SYNGR3 contribute to the neurotransmitter release in neurons by interactions with the GABA and VGLUT transporters in primary neurons and in C. elegans (4-6). SYNGRs are associated with disease including Schizophrenia (7,8) and Alzheimer's disease (9,10).
- Kedra, D. et al. (1998) Hum Genet 103, 131-41.
- Janz, R. et al. (1999) Neuron 24, 687-700.
- Sugita, S. et al. (1999) J Biol Chem 274, 18893-901.
- Belizaire, R. et al. (2004) J Comp Neurol 470, 266-81.
- Bragina, L. et al. (2010) Neuroscience 165, 934-43.
- Abraham, C. et al. (2011) Neuroscience 190, 75-88.
- Verma, R. et al. (2005) J Hum Genet 50, 635-40.
- Cheng, M.C. and Chen, C.H. (2007) J Psychiatr Res 41, 1027-31.
- Liu, C. et al. (2016) J Biol Chem 291, 8173-88.
- McInnes, J. et al. (2018) Neuron 97, 823-835.e8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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