WB, IP
H M R Hm Mk
Endogenous
45
Rabbit IgG
#P17706-2
5771
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro382 of human TCPTP (TC45) protein.
Background
T cell protein tyrosine phosphatase (TCPTP, PTPN2, PTN2) is a non-receptor protein tyrosine phosphatase (PTP) that regulates signal transduction pathways by catalyzing the dephosphorylation of tyrosine residues (1). Two described TCPTP splice variants include a 48 kDa isoform (TC48) that is targeted to secretory pathway organelles (e.g., endoplasmic reticulum) by a hydrophobic carboxy terminus, and a 45 kDa isoform (TC45) that actively shuttles between the nucleus and cytoplasm (2). TCPTP substrates include receptor and non-receptor tyrosine kinases, such as EGFR, JAK1/3, and Src-family kinases, as well as STAT3 and other nuclear substrates (3-6). Research studies show that the corresponding PTPN2 gene is deleted in a subset of human T-cell acute lymphoblastic leukemias. The loss of TCPTP has been suggested to promote tumor progression through enhanced JAK/STAT signaling (7,8).
- Muppirala, M. et al. (2013) Biochim Biophys Acta 1833, 1125-32.
- Lorenzen, J.A. et al. (1995) J Cell Biol 131, 631-43.
- Simoncic, P.D. et al. (2002) Curr Biol 12, 446-53.
- Tiganis, T. et al. (1998) Mol Cell Biol 18, 1622-34.
- van Vliet, C. et al. (2005) Nat Immunol 6, 253-60.
- Fukushima, A. et al. (2010) Diabetes 59, 1906-14.
- Kleppe, M. et al. (2010) Nat Genet 42, 530-5.
- Kleppe, M. et al. (2011) Blood 117, 7090-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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