WB
H M
Endogenous
13
Rabbit
#P04155
7031
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human TFF1/pS2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The trefoil factor (TFF) family of proteins (TFF1/pS2, TFF2, and TFF3) are a group of highly conserved, secreted polypeptides that are expressed by mucus-secreting cells of the gastrointestinal tract. Within the gastrointestinal tract, TFFs display both common and distinct expression patterns (1). Collectively, the TFF family of proteins play a prominant role in the protection and repair of the mucous epithelia lining the gastrointestinal tract through their interactions with mucins (2). TFFs have been shown to regulate a number of cellular processes such as migration, apoptosis, and proliferation. In humans, dysregulated expression of TFFs has been observed in inflammatory bowel diseases as well as tumors of the breast, colon, lung, and stomach (2).
TFF1 is expressed predominantly by the gastric epithelia, in the upper portion of the glandular pits, and is highly expressed in some adenocarcinomas such as breast cancer (1,3,4). In the context of breast cancer, TFF1 is highly expressed in estrogen receptor-positive tumors. Indeed, TFF1 expression is directly regulated by estrogen receptor-α (4). In the stomach, secreted TFF1 is a component of the protective mucous layer. TFF1 expression is strongly induced after mucosal injury (5) and is involved in stomach ontogenesis and maintenance of the integrity of the mucosa (1,3). Research studies have shown frequent loss of TFF1 expression in more than two-thirds of gastric carcinomas resulting from mutation-independent mechanisms (6-8).
- Madsen, J. et al. (2007) J Histochem Cytochem 55, 505-13.
- Taupin, D. and Podolsky, D.K. (2003) Nat Rev Mol Cell Biol 4, 721-32.
- Ribieras, S. et al. (1998) Biochim Biophys Acta 1378, F61-77.
- Corte, M.D. et al. (2006) Breast Cancer Res Treat 96, 63-72.
- Taupin, D. et al. (2001) Lab Invest 81, 397-408.
- Carvalho, R. et al. (2002) Lab Invest 82, 1319-26.
- Katoh, M. (2003) Int J Mol Med 12, 3-9.
- McChesney, P.A. et al. (2006) Cancer Res 66, 1346-53.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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