WB, IHC-P
H
Endogenous
13
Rabbit IgG
#P04155
7031
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:150 |
Storage
For a carrier free (BSA and azide free) version of this product see product #72033.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human TFF1/pS2 protein.
Background
The trefoil factor (TFF) family of proteins (TFF1/pS2, TFF2, and TFF3) are a group of highly conserved, secreted polypeptides that are expressed by mucus-secreting cells of the gastrointestinal tract. Within the gastrointestinal tract, TFFs display both common and distinct expression patterns (1). Collectively, the TFF family of proteins play a prominant role in the protection and repair of the mucous epithelia lining the gastrointestinal tract through their interactions with mucins (2). TFFs have been shown to regulate a number of cellular processes such as migration, apoptosis, and proliferation. In humans, dysregulated expression of TFFs has been observed in inflammatory bowel diseases as well as tumors of the breast, colon, lung, and stomach (2).
TFF1/pS2 is a gastric peptide that is highly expressed by mucosal goblet cells of the stomach, where it is thought to play a role in maintaining the integrity of the epithelial layer of the mucosa through the regulation of cell-cell adhesion and cell migration (2,3). Research studies have shown that TFF1 functions as a tumor suppressor in the stomach as its expression is frequently lost in gastric carcinomas, largely due to promoter hypermethylation (4-8). Research studies have also demonstrated that TFF1 is a transcriptional target of estrogen receptor-α and that TFF1 expression in breast carcinoma may be used as a predictive biomarker for response to anti-estrogen therapy (9,10).
- Madsen, J. et al. (2007) J Histochem Cytochem 55, 505-13.
- Taupin, D. and Podolsky, D.K. (2003) Nat Rev Mol Cell Biol 4, 721-32.
- Taupin, D. et al. (2001) Lab Invest 81, 397-408.
- Carvalho, R. et al. (2002) Lab Invest 82, 1319-26.
- Katoh, M. (2003) Int J Mol Med 12, 3-9.
- McChesney, P.A. et al. (2006) Cancer Res 66, 1346-53.
- Mashimo, H. et al. (1996) Science 274, 262-5.
- Lefebvre, O. et al. (1996) Science 274, 259-62.
- May, F.E. and Westley, B.R. (2015) Endocr Relat Cancer 22, 465-79.
- Corte, M.D. et al. (2006) Breast Cancer Res Treat 96, 63-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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