WB
H
Endogenous
62, 55
Rabbit IgG
#Q9NRS4
56649
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMPRSS4 protein.
Background
Transmembrane protease serine 4 (TMPRSS4) is a type-II transmembrane glycoprotein that functions as a serine protease. The extracellular region of TMPRSS4 harbors a serine protease catalytic domain and a scavenger receptor domain, which is implicated in lipid binding (1,2). TMPRSS4 activity has been implicated in viral infections through its ability to regulate the proteolytic processing of the influenza virus hemagglutinin protein and SARS-CoV-2 spike protein (3,4). TMPRSS4 is also implicated in cancer cell metastasis through the cleavage of urokinase-type plasminogen activator and activation of intracellular signaling cascades that promote cell migration (5,6). These findings, combined with the observation that TMPRSS4 is overexpressed in multiple types of human solid tumors, have prompted investigation into therapeutically targeting TMPRSS4 protease activity (6-9).
- Hooper, J.D. et al. (2001) J Biol Chem 276, 857-60.
- Netzel-Arnett, S. et al. (2003) Cancer Metastasis Rev 22, 237-58.
- Bertram, S. et al. (2010) Rev Med Virol 20, 298-310.
- Zang, R. et al. (2020) Sci Immunol 5, eabc3582. doi: 10.1126/sciimmunol.abc3582.
- Min, H.J. et al. (2014) Biochem Biophys Res Commun 446, 1-7.
- Kim, S. et al. (2010) Carcinogenesis 31, 597-606.
- Wallrapp, C. et al. (2000) Cancer Res 60, 2602-6.
- Luo, Z.Y. et al. (2013) PLoS One 8, e70311.
- Kang, S. et al. (2013) Bioorg Med Chem Lett 23, 1748-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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