IHC-Bond, IHC-P
H M
Endogenous
Rabbit IgG
#Q15661
7177
Product Information
Product Usage Information
This product is the carrier free version of product #19523. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Tryptase protein.
Background
Tryptase is the most abundant neutral serine protease expressed in the secretory granules of all human mast cells (1). Tryptase is secreted upon the coupled activation-degranulation response of mast cells in peripheral tissues to physical factors such as trauma, toxins, venoms, endogenous mediators, and immune mechanisms (IgE-dependent and IgE-independent) (2). Tryptase has distinct enzymatic functions that depend on the monomeric or homotetrameric state of this protein, the pH of the environment, and the presence or absence of heparin (3-5). Tryptase has the ability to cleave extracellular substrates such as vasoactive intestinal peptides (6), calcitonin gene-related peptides (7), fibronectins (8), fibrinogens (3), and kininogens (9). Tryptase is also a potent growth factor for epithelial cells, airway smooth muscle cells, and fibroblasts (10-13).
- Vanderslice, P. et al. (1990) Proc Natl Acad Sci U S A 87, 3811-5.
- Krishnaswamy, G. et al. (2001) Front Biosci 6, D1109-27.
- Fukuoka, Y. and Schwartz, L.B. (2007) Int Immunopharmacol 7, 1900-8.
- Ren, S. et al. (1998) J Immunol 160, 4561-9.
- Sakai, K. et al. (1996) J Clin Invest 97, 988-95.
- Tam, E.K. and Caughey, G.H. (1990) Am J Respir Cell Mol Biol 3, 27-32.
- Walls, A.F. et al. (1992) Biochem Pharmacol 43, 1243-8.
- Lohi, J. et al. (1992) J Cell Biochem 50, 337-49.
- Walls, A.F. et al. (1992) Biochem Soc Trans 20, 260S.
- Cairns, J.A. and Walls, A.F. (1996) J Immunol 156, 275-83.
- Ruoss, S.J. et al. (1991) J Clin Invest 88, 493-9.
- Brown, J.K. et al. (1995) Am J Respir Cell Mol Biol 13, 227-36.
- Gruber, B.L. et al. (1997) J Immunol 158, 2310-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
