WB
H M R Mk
Endogenous
135
Rabbit
#Q15361
7270
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His34 of human TTF-I protein. Antibodies are purified by peptide affinity chromatography.
Background
Transcription termination factor I (TTF-I) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF-I binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3’ ends of the genes. Binding of TTF-I to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC (1-5). TTF-I also has an activating function by binding an upstream terminator element to induce chromatin remodeling (6). TTF-I localization can be controlled by key tumor suppressor ARF, which binds to the TTF-I nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis (7). Furthermore, TTF-I can be ubiquitinylated by MDM2, which targets it for degradation (8). TTF-I has also been identified to play a role in HIV-1 replication (9).
- Bartsch, I. et al. (1988) Mol Cell Biol 8, 3891-7.
- Evers, R. and Grummt, I. (1995) Proc Natl Acad Sci U S A 92, 5827-31.
- Lang, W.H. et al. (1994) Cell 79, 527-34.
- Mason, S.W. et al. (1997) J Mol Biol 268, 229-34.
- Strohner, R. et al. (2004) Mol Cell Biol 24, 1791-8.
- Längst, G. et al. (1997) EMBO J 16, 760-8.
- Lessard, F. et al. (2010) Mol Cell 38, 539-50.
- Lessard, F. et al. (2012) Nucleic Acids Res 40, 5357-67.
- Park, S.H. et al. (2018) BMB Rep 51, 338-343.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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