WB, IP
H M R Mk
Endogenous
70
Rabbit IgG
#Q9UHD9
29978
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN2 protein.
Background
Ubiquilin 2 (UBQLN2) is a broadly expressed member of the ubiquilin family of ubiquitin receptor proteins. UBQLN2 is a type 2 ubiquitin-like (UBL) protein that contains an amino-terminal UBL domain, multiple heat shock chaperonin-binding (STI) motifs, several PXX repeats, and a carboxy-terminal ubiquitin-associated (UBA) domain (1-3). Research studies indicate that the UBL domain of UBQLN2 can interact with proteasome subunits (4). The UBA domain of UBQLN2 can interact with ubiquitinated proteins and the autophagosome and allows UBQLN2 to participate in the ubiquitin-proteasome and autophagy pathways (5-8). Mutations in the PXX repeat region of the corresponding UBQLN2 gene are associated with an X-linked form of amyotrophic lateral sclerosis (ALS15) and dementia with reduced penetrance in females (9).
- Wu, A.L. et al. (1999) Mol Cell 4, 619-25.
- Kaye, F.J. et al. (2000) FEBS Lett 467, 348-55.
- Mah, A.L. et al. (2000) J Cell Biol 151, 847-62.
- Kleijnen, M.F. et al. (2000) Mol Cell 6, 409-19.
- Wilkinson, C.R. et al. (2001) Nat Cell Biol 3, 939-43.
- N'Diaye, E.N. et al. (2009) EMBO Rep 10, 173-9.
- N'Diaye, E.N. et al. (2009) Autophagy 5, 573-5.
- Lee, D.Y. et al. (2013) EMBO Rep 14, 373-81.
- Deng, H.X. et al. (2011) Nature 477, 211-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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