WB, IP
H M R
Endogenous
42
Rabbit IgG
#Q04323
51035
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu101 of human UBXN1 protein.
Background
Ubiquitin regulatory X (UBX) domain-containing protein 1 (UBXN1) is a modular ubiquitin binding protein that consists of three domains: an N-terminal ubiquitin-associated (UBA) domain, a central coiled-coil domain, and a C-terminal UBX domain (1). UBXN1 has been shown to modulate the host innate immune response to viral infection. Indeed, research studies have demonstrated that UBXN1 retrains the type I interferon response induced by RNA viruses by binding to MAVS and compromising its oligomerization (2). UBXN1 has also been shown to negatively regulate NF-κB activation in response to proinflammatory cytokines, a finding which may have implications for antiviral signaling cascades (3). In addition to regulating antiviral signaling cascades, research has shown that UBXN1 participates in protein quality control by interacting with p97/VCP (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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