WB, IP
H M R
Endogenous
130
Rabbit IgG
#Q8IZJ1
219699
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Xenopus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly420 of human UNC5B protein.
Background
Netrin proteins belong to an evolutionarily conserved family of laminin-like molecules that are involved in axon guidance and vascular development. These secreted proteins can have opposing functions depending on specific receptor association. For example, deleted in colorectal cancer (DCC) family receptors typically mediate cellular attraction (1,2) while netrin bound to UNC5 family receptors induce cellular repulsion (2-4). The uncoordinated 5B homolog (UNC5B) is a transmembrane protein with extracellular Ig-like domains and an intracellular region containing a protein-binding death domain and a putative DCC interaction domain (2). Homodimers composed of DCC receptor proteins mediate axonal attraction responses, while UNC5B homodimers and UNC5B-DCC heterodimers promote cellular repulsion (2). The netrin receptor UNC5B mediates apoptosis in the absence of netrin through the activation of DAP kinase (5) and is involved in leukocyte migration inhibition (6). Expression of UNC5B correlates with bladder cancer stage and the receptor is a potential predictor of both bladder and colorectal cancer prognosis and possible disease recurrence (7,8).
- Keino-Masu, K. et al. (1996) Cell 87, 175-85.
- Hong, K. et al. (1999) Cell 97, 927-41.
- Leung-Hagesteijn, C. et al. (1992) Cell 71, 289-99.
- Lu, X. et al. (2004) Nature 432, 179-86.
- Guenebeaud, C. et al. (2010) Mol Cell 40, 863-76.
- Ly, N.P. et al. (2005) Proc Natl Acad Sci U S A 102, 14729-34.
- Liu, J. et al. (2013) Tumour Biol 34, 2099-108.
- Okazaki, S. et al. (2012) Int J Oncol 40, 209-16.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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