WB, IF-F, IF-IC
H M R
Endogenous
27
Rabbit IgG
#O95292
9217
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAPB protein.
Background
The human VAP family proteins, which include VAPA (also known as VAP33), VAPB, and VAPC, were initially identified as homologues of vesicle-associated membrane protein (VAMP)-associated protein (VAP) that is involved in exocytosis of neurotransmitters (1). VAPB is an integral endoplasmic reticulum (ER) protein whose amino terminus projects into the cytosol. A proline to serine substitution at position-56 (VAPBP56S) leads to an autosomal-dominant form of amyotrophic lateral sclerosis (ALS), classified as ALS-8. (2). VAPB has been implicated in a variety of processes, including ER stress and the unfolded protein response (UPR), ER to Golgi transport, and bouton formation at the neuromuscular junction (1,2). The P56S mutation causes almost complete loss of function of VAPB to mediate UPR by inducing its misfolding and localization shift to non-ER compartments (1). A possible role of VAPB as a pathologic marker in PBMCs from ALS patients has been published (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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