WB
H
Endogenous
42
Rabbit IgG
#Q9Y5W5
11197
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro168 of human WIF1 protein.
Background
WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8).
- Hsieh, J.C. et al. (1999) Nature 398, 431-6.
- Liepinsh, E. et al. (2006) J Mol Biol 357, 942-50.
- Gorfinkiel, N. et al. (2005) Dev Cell 8, 241-53.
- Glise, B. et al. (2005) Dev Cell 8, 255-66.
- Wissmann, C. et al. (2003) J Pathol 201, 204-12.
- Taniguchi, H. et al. (2005) Oncogene 24, 7946-52.
- Mazieres, J. et al. (2004) Cancer Res 64, 4717-20.
- Mazieres, J. et al. (2005) Cancer Lett 222, 1-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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