WB
H
Endogenous
51
Rabbit IgG
#P19544
7490
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val306 of human WT1 protein.
Background
Wilms' Tumor 1 (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the WT1 gene (1). It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of expression of WT1 result in a variety of syndromes affecting the genitourinary system and other tissues (2-5).
WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post translation modifications of the protein (9).
- Royer-Pokora, B. et al. (2004) Am J Med Genet A 127A, 249-57.
- Kohsaka, T. et al. (1999) Hum Mutat 14, 466-70.
- Little, M. et al. (2000) Hum Mutat 15, 389.
- Takata, A. et al. (2000) J Med Genet 37, 698-701.
- Suri, M. et al. (2007) Am J Med Genet A 143A, 2312-20.
- Toska, E. and Roberts, S.G. (2014) Biochem J 461, 15-32.
- Yang, L. et al. (2007) Leukemia 21, 868-76.
- Weiss, T.C. and Romaniuk, P.J. (2009) Biochemistry 48, 148-55.
- Haber, D.A. et al. (1991) Proc Natl Acad Sci U S A 88, 9618-22.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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