WB
H R
Endogenous
51
Rabbit
#Q9H5U6
29063
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZCCHC4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
N6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. Zinc finger CCHC domain-containing protein 4 (ZCCHC4) is an rRNA m6A methyltransferase that specifically carries out m6A methylation of 28S rRNA at position 4220 (1). ZCCHC4 is highly conserved among multicellular organisms, and knockout of ZCCHC4 ablates m6A methylation exclusively at position 4420 on 28s rRNA, suggesting that this is an evolutionarily conserved function (1,2). Knockout of ZCCHC4, and consequently the m6A 4220 modification, has been reported to reduce overall protein translation and inhibit cell proliferation, while others have reported that this knockout had no effect on cell proliferation nor rRNA production (1,2). ZCCHC4 has been found to be overexpressed in hepatocellular carcinoma, and ZCCHC4 knockout cells showed reduced tumor growth in a xenograft mouse model (1). Interestingly, X-ray crystallography reveals that ZCCHC4 contains an autoinhibitory regulatory loop that blocks its catalytic site, a unique feature amongst m6A RNA methyltransferases currently identified (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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