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ZCCHC4 Antibody

ZCCHC4 Antibody #64933

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Supporting Data

REACTIVITY	H R
SENSITIVITY	Endogenous
MW (kDa)	51
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ZCCHC4 Antibody recognizes endogenous levels of total ZCCHC4 protein.

Species Reactivity:

Human, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZCCHC4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

N6-methyladenosine (m6A) is a post-transcriptional modification found in various RNA subtypes. Zinc finger CCHC domain-containing protein 4 (ZCCHC4) is an rRNA m6A methyltransferase that specifically carries out m6A methylation of 28S rRNA at position 4220 (1). ZCCHC4 is highly conserved among multicellular organisms, and knockout of ZCCHC4 ablates m6A methylation exclusively at position 4420 on 28s rRNA, suggesting that this is an evolutionarily conserved function (1,2). Knockout of ZCCHC4, and consequently the m6A 4220 modification, has been reported to reduce overall protein translation and inhibit cell proliferation, while others have reported that this knockout had no effect on cell proliferation nor rRNA production (1,2). ZCCHC4 has been found to be overexpressed in hepatocellular carcinoma, and ZCCHC4 knockout cells showed reduced tumor growth in a xenograft mouse model (1). Interestingly, X-ray crystallography reveals that ZCCHC4 contains an autoinhibitory regulatory loop that blocks its catalytic site, a unique feature amongst m6A RNA methyltransferases currently identified (3).

Database Links

UniProt ID: Q9H5U6

Entrez-Gene Id: 29063

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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