Product Information
Product Usage Information
PTMScan® Trypsin, Mass Spectrometry Grade is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the Trypsin Digestion of Enriched Peptides step. Reconstitute the lyophilized trypsin with 40ul of mass spectrometry grade water to make a 0.5 ug/ul stock. Use immediately or aliquot and store at -80°C. Consult the specific PTMScan® kit and protocol for more details.
Storage
Source / Purification
Isolated from bovine (Bos taurus) pancreas.
Product Description
Background
Trypsin digests polypeptides by hydrolysis at the carboxyl side of unmodified arginine and lysine residues (1). Proteolysis is slower when the cleavage site is flanked by acidic residues and will not occur if the lysine or arginine is followed by a proline (2-5). Trypsin's activity is optimal at pH 8.0 and is inhibited to varying degrees by organophosphorus compounds such as diisopropyl fluorophosphate, as well as EDTA, aprotinin, Ag+, and benzamidine (6-8).
- Brown, W.E. and Wold, F. (1973) Biochemistry 12, 828-34.
- Rodriguez, J. et al. (2008) J Proteome Res 7, 300-5.
- Leiros, H.K. et al. (2004) Protein Sci 13, 1056-70.
- Rawlings, N.D. and Barrett, A.J. (1994) Methods Enzymol 244, 19-61.
- Perona, J.J. and Craik, C.S. (1995) Protein Sci 4, 337-60.
- KOSTKA, V. and CARPENTER, F.H. (1964) J Biol Chem 239, 1799-803.
- Levilliers, N. et al. (1970) Arch Biochem Biophys 140, 474-83.
- Polgár, L. (2005) Cell Mol Life Sci 62, 2161-72.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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