#P14373
5987
Product Information
Product Usage Information
CST recommends transfection with 100 nM SignalSilence® TRIM27 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Product Description
Background
Tripartite motif containing protein 27 (TRIM27, RFP) is a member of the tripartite motif (TRIM) family whose members contain a RING domain, a B-box, and a coiled-coil region (together called RBCC). TRIM27 was originally discovered as part of an oncogenic DNA rearrangement resulting in a fusion of the amino terminal RBCC region of TRIM27 with the carboxyl terminal kinase domain of the receptor tyrosine kinase Ret (1). Overexpression of TRIM27 induces JNK and p38 MAPK activation as well as apoptosis (2). TRIM27 has been found to have pleiotropic effects including transcriptional repression (3,4), and E3 ligase activity for ubiquitin (5-7), and SUMO (8). TRIM27 was originally found to interact with Enhancer of Polycomb (EPC) and function as a transcriptional repressor (3). Subsequent studies have identified ubiquitin E3 ligase activity in TRIM27 as well as other members of the TRIM family (reviewed in 9). Potential substrates of TRIM27-mediated ubiquitination include class II PI3K-C2β, NOD2, and WASH. Elevated expression of TRIM27 has been observed in several types of cancer, where in some cases it may be a predictor of poor prognosis (10-13).
- Takahashi, M. et al. (1988) Mol Cell Biol 8, 1853-6.
- Dho, S.H. and Kwon, K.S. (2003) J Biol Chem 278, 31902-8.
- Shimono, Y. et al. (2000) J Biol Chem 275, 39411-9.
- Bloor, A.J. et al. (2005) Oncogene 24, 6729-36.
- Cai, X. et al. (2011) Proc Natl Acad Sci U S A 108, 20072-7.
- Zurek, B. et al. (2012) PLoS One 7, e41255.
- Hao, Y.H. et al. (2013) Cell 152, 1051-64.
- Chu, Y. and Yang, X. (2011) Oncogene 30, 1108-16.
- Meroni, G. and Diez-Roux, G. (2005) Bioessays 27, 1147-57.
- Tezel, G.G. et al. (2009) Pathol Res Pract 205, 403-8.
- Tsukamoto, H. et al. (2009) Cancer Sci 100, 1895-901.
- Iwakoshi, A. et al. (2012) Pathol Int 62, 324-30.
- Zoumpoulidou, G. et al. (2012) J Natl Cancer Inst 104, 941-52.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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