Western Blot Stripping Buffer (5X) (#91925) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Storage

Store Western Blot Stripping Buffer (5X) at room temperature. This product is stable for at least 12 months.

Product Description

Western Blot Stripping Buffer (5X) allows for quick and effective removal of both primary and secondary antibodies from nitrocellulose membranes. This can be done without damaging the immobilized antigen, enabling the membranes to be reprobed with alternative antibodies or concentrations for further western blot analysis. This product should be diluted to 1X using RODI-water, and 2-mercaptoethanol should be added right before use. Completely submerge the membrane in Western Blot Stripping Buffer (1X). Optimization of incubation time or temperature may be required to achieve desired results.

Background

Having to re-run gels and repeat western blotting efforts is costly and time-consuming. Western Blot Stripping Buffer (5X) enables the membranes to be reused with the added benefit of not having to re-load expensive or limited samples into the gel. Allowing for the detection of a different target with an alternative primary antibody or by reprobing with a different concentration of a primary antibody saves valuable time.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.