Render Target: STATIC
Render Timestamp: 2024-10-09T09:40:46.303Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

Western Blot Stripping Buffer (5X) #91925

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    Product Information

    Storage

    Store Western Blot Stripping Buffer (5X) at room temperature. This product is stable for at least 12 months.

    Protocol

    Product Description

    Western Blot Stripping Buffer (5X) allows for quick and effective removal of both primary and secondary antibodies from nitrocellulose membranes. This can be done without damaging the immobilized antigen, enabling the membranes to be reprobed with alternative antibodies or concentrations for further western blot analysis. This product should be diluted to 1X using RODI-water, and 2-mercaptoethanol should be added right before use. Completely submerge the membrane in Western Blot Stripping Buffer (1X). Optimization of incubation time or temperature may be required to achieve desired results.

    Background

    Having to re-run gels and repeat western blotting efforts is costly and time-consuming. Western Blot Stripping Buffer (5X) enables the membranes to be reused with the added benefit of not having to re-load expensive or limited samples into the gel. Allowing for the detection of a different target with an alternative primary antibody or by reprobing with a different concentration of a primary antibody saves valuable time.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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      U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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