Background
Phorbol 12, 13-Dibutyrate (PDBu) is a phorbol ester and a potent activator of protein kinase C (PKC). Frequently used in cancer research, PDBu is widely characterized to promote tumors in a variety of tissue types (1,2). PDBu treatment of cells can lead to activation of downstream PKC targets such as p44/42 MAPK and MARCKS (3-5). Although less potent than TPA, research has shown PDBu elicits less nonspecific binding (6,7) and is less hydrophobic allowing for easy wash out from cells in tissue culture (8).
- Blumberg, P.M. et al. (1983) Ann N Y Acad Sci 407, 303-15.
- Driedger, P.E. and Blumberg, P.M. (1980) Proc Natl Acad Sci U S A 77, 567-71.
- Caunt, C.J. and McArdle, C.A. (2010) J Cell Sci 123, 4310-20.
- Bapat, S. et al. (2001) FEBS Lett 499, 21-6.
- Tanabe, A. et al. (2012) J Cell Physiol 227, 618-29.
- Shoyab, M. and Todaro, G.J. (1980) Nature 288, 451-5.
- Chida, K. and Kuroki, T. (1983) Cancer Res 43, 3638-42.
- Dunphy, W.G. et al. (1981) Cancer Res 41, 2640-7.
Molecular Formula
C28H40O8
Molecular Weight
504.61 g/mol
Purity
>99%
CAS
37558-16-0
Solubility
Souble in DMSO at 25mg/ml and EtOH at 20mg/ml.
Storage
Directions for Use
PDBu is supplied as a lyophilized powder. For a 2 mM stock, reconstitute the 1 mg in 990.9 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 20-2000 nM for 15-30 min. Soluble in DMSO or ethanol.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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