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12808
PDBu (Phorbol 12,13-Dibutyrate)

PDBu (Phorbol 12,13-Dibutyrate) #12808

Western Blotting

Western blot analysis of extracts from HeLa cells serum-starved overnight, and untreated (-) or treated with PDBu (15 min; +) at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).

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Structure

Chemical structure of PDBu.

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PDBu is supplied as a lyophilized powder. For a 2 mM stock, reconstitute the 1 mg in 990.9 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 20-2000 nM for 15-30 min. Soluble in DMSO or ethanol.

Storage:

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

504.61 g/mol

Purity:

>99%

Molecular Formula:

C28H40O8

Phorbol 12, 13-Dibutyrate (PDBu) is a phorbol ester and a potent activator of protein kinase C (PKC). Frequently used in cancer research, PDBu is widely characterized to promote tumors in a variety of tissue types (1,2). PDBu treatment of cells can lead to activation of downstream PKC targets such as p44/42 MAPK and MARCKS (3-5). Although less potent than TPA, research has shown PDBu elicits less nonspecific binding (6,7) and is less hydrophobic allowing for easy wash out from cells in tissue culture (8).

  1. Blumberg, P.M. et al. (1983) Ann N Y Acad Sci 407, 303-15.
  2. Driedger, P.E. and Blumberg, P.M. (1980) Proc Natl Acad Sci U S A 77, 567-71.
  3. Caunt, C.J. and McArdle, C.A. (2010) J Cell Sci 123, 4310-20.
  4. Bapat, S. et al. (2001) FEBS Lett 499, 21-6.
  5. Tanabe, A. et al. (2012) J Cell Physiol 227, 618-29.
  6. Shoyab, M. and Todaro, G.J. (1980) Nature 288, 451-5.
  7. Chida, K. and Kuroki, T. (1983) Cancer Res 43, 3638-42.
  8. Dunphy, W.G. et al. (1981) Cancer Res 41, 2640-7.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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To Purchase # 12808S

Product Number Size Price
12808S 1 mg $102.00.0
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