WB
H
Transfected Only
77
Rabbit IgG
#Q155Q3
85458
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu579 of human DIXDC1 protein.
Background
DIXDC1 (DIXIN, CCD1), is a Disheveled-Axin (DIX) domain protein that was first identified in zebrafish, where it was shown to positively regulate the canonical Wnt signaling pathway (1). In vitro studies subsequently showed that DIXDC1 activates Wnt signaling by binding to Disheveled (Dvl), resulting in the conversion of Dvl from a (latent) polymeric form to biologically active oligomers (2). In vivo studies in mice confirmed an important role for DIXDC1 in early development, by showing that DIXDC1 regulates both neural progenitor proliferation and neuronal migration, via Wnt-dependent and Wnt-independent mechanisms, respectively (3). Additional research suggests that DIXDC1 may function as a suppressor of metastasis by negatively regulating Snail1, implicating DIXDC1 in regulation of epithelial-mesenchymal transition (4). This function for DIXDC1 was shown to be dependent upon phosphorylation at Ser592 by MARK1 and MARK4 kinases (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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