Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
DIXDC1 (D7P6Q) Rabbit mAb recognizes transfected levels of total DIXDC1 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu579 of human DIXDC1 protein.
DIXDC1 (DIXIN, CCD1), is a Disheveled-Axin (DIX) domain protein that was first identified in zebrafish, where it was shown to positively regulate the canonical Wnt signaling pathway (1). In vitro studies subsequently showed that DIXDC1 activates Wnt signaling by binding to Disheveled (Dvl), resulting in the conversion of Dvl from a (latent) polymeric form to biologically active oligomers (2). In vivo studies in mice confirmed an important role for DIXDC1 in early development, by showing that DIXDC1 regulates both neural progenitor proliferation and neuronal migration, via Wnt-dependent and Wnt-independent mechanisms, respectively (3). Additional research suggests that DIXDC1 may function as a suppressor of metastasis by negatively regulating Snail1, implicating DIXDC1 in regulation of epithelial-mesenchymal transition (4). This function for DIXDC1 was shown to be dependent upon phosphorylation at Ser592 by MARK1 and MARK4 kinases (4).
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