WB, IP
H M R
Endogenous
50
Rabbit IgG
#P78414
79192
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro466 of human IRX1 protein.
Background
The Iroquois-class homeodomain proteins (IRX1-6) comprise a family of transcription factors, initially identified in a mutagenesis experiment in Drosophila designed to identify genes involved in embryonic patterning (1,2). Members of the Iroquois-class protein family contain a three amino acid loop extension (TALE) homeodomain and an IRO sequence motif of unknown function that is unique to this protein family, but which bears similarity to Notch receptor domains involved in protein-protein interactions (3,4). Research studies in various animal models suggest important roles for IRX proteins in early development, including neural patterning, heart field specification, kidney development, and lung development (5-8). Moreover, studies in mice have shown an essential role for IRX proteins in eye development, where they are required for the specification of ommatidia and photoreceptor cells (9).
The human IRX1 gene has been identified as a candidate tumor suppressor (10) that is hypermethylated in a number of cancer subtypes (11). Research studies in head and neck squamous cell carcinoma cell lines specifically suggest that IRX1 interacts with components of the TGFβ signaling pathway to regulate cell proliferation and differentiation (12).
- Dambly-Chaudière, C. and Leyns, L. (1992) Int J Dev Biol 36, 85-91.
- Leyns, L. et al. (1996) Mech Dev 59, 63-72.
- Cavodeassi, F. et al. (2001) Development 128, 2847-55.
- Lai, E.C. et al. (1998) Development 125, 4077-88.
- Bosse, A. et al. (1997) Mech Dev 69, 169-81.
- Christoffels, V.M. et al. (2000) Dev Biol 224, 263-74.
- Marra, A.N. and Wingert, R.A. (2014) Cell Dev Biol 3, 1000131.
- Becker, M.B. et al. (2001) Mech Dev 106, 155-8.
- Choy, S.W. et al. (2010) Dev Dyn 239, 3204-14.
- Mahoney, S.E. et al. (2012) Epigenetics 7, 400-8.
- Bennett, K.L. et al. (2008) Cancer Res 68, 4494-9.
- Bennett, K.L. et al. (2009) Cancer Res 69, 9301-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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