Revision 4

#68030Store at +4C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IHC-Bond, IHC-P

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Mouse IgG1

Product Information

Product Usage Information

Application Dilution
IHC Leica Bond 1:400 - 1:1600
Immunohistochemistry (Paraffin) 1:800 - 1:3200

Storage

Supplied in PBS containing 1% BSA and 15 mM sodium azide. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

Specificity / Sensitivity

CA 19-9 (C241:5:1:4) Mouse mAb recognizes endogenous levels of CA 19-9 protein. This antibody has minimal cross-reactivity (<1%) with sialyl lacto-N-tetraosylceramide. This antibody does not cross-react with Lewis A, Lewis B, or structurally-related substances.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human colorectal adenocarcinoma Colo 205 cells.

Background

CA 19-9 specifically binds to the epitope of sialylated Lewis A carbohydrate structure, which is the functional ligand for E-selectin and is involved in the adhesion of cancer cells (1,2). The CA 19-9 antigen has been of clinical interest in detection, diagnosis, and management of cancers such as pancreatic and gastrointestinal carcinomas (3,4).

  1. Itzkowitz, S.H. and Kim, Y.S. (1986) Gastroenterology 90, 491-4.
  2. Ugorski, M. and Laskowska, A. (2002) Acta Biochim Pol 49, 303-11.
  3. Goggins, M. (2005) J Clin Oncol 23, 4524-31.
  4. Herlyn, M. et al. (1982) J Clin Immunol 2, 135-40.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 4
#68030

CA 19-9 (C241:5:1:4) Mouse mAb

Immunohistochemistry Image 1: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CA 19-9 (C241:5:1:4) Mouse mAb performed on the Leica® BOND Rx.
Immunohistochemistry Image 2: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CA 19-9 (C241:5:1:4) Mouse mAb performed on the Leica® BOND Rx.
Immunohistochemistry Image 3: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human pancreas using CA 19-9 (C241:5:1:4) Mouse mAb performed on the Leica® BOND Rx.
Immunohistochemistry Image 1: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CA 19-9 (C241:5:1:4) Mouse mAb.
Immunohistochemistry Image 2: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using CA 19-9 (C241:5:1:4) Mouse mAb.
Immunohistochemistry Image 3: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using CA 19-9 (C241:5:1:4) Mouse mAb.
Immunohistochemistry Image 4: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human colon using CA 19-9 (C241:5:1:4) Mouse mAb.
Immunohistochemistry Image 5: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human kidney using CA 19-9 (C241:5:1:4) Mouse mAb.
Immunohistochemistry Image 6: CA 19-9 (C241:5:1:4) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human small intestine using CA 19-9 (C241:5:1:4) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).