WB
H M R
Endogenous
70, 80, 130, 247
Rabbit
#Q9BTC0
11083
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr160 of human DIDO1. Antibodies were purified by protein A and peptide affinity chromatography.
Background
The putative transcription factor DIDO1 (death inducer obliterator 1, also termed DIO-1 or DATF1) contains a pair of zinc finger motifs and is upregulated by apoptotic stimuli. DIDO1 is expressed in the developing limb and may play a role in controlling programmed cell death during development (1-3). Nuclear translocation of DIDO1 during apoptosis is associated with its apoptotic activity (2). Alternative splicing produces the DIDO-1, -2 and -3 isoforms (also termed DIO-1, -2, -3), whose targeted disruption in mice produces a phenotype similar to myelodysplastic/myeloproliferative disease (MPS/MPD) in humans (3). DIDO3, the largest of the splice variants, is associated with the centrosome and plays a role in mitotic checkpoint and chromosome stability (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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