Render Target: STATIC
Render Timestamp: 2024-10-09T09:47:46.879Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DIDO1 Antibody #3605

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 70, 80, 130, 247
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DIDO1 Antibody detects endogenous levels of DIDO1 and other isoforms including DIDO2 and DIDO3. An unknown band is detected in HepG2 cells at 35 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr160 of human DIDO1. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    The putative transcription factor DIDO1 (death inducer obliterator 1, also termed DIO-1 or DATF1) contains a pair of zinc finger motifs and is upregulated by apoptotic stimuli. DIDO1 is expressed in the developing limb and may play a role in controlling programmed cell death during development (1-3). Nuclear translocation of DIDO1 during apoptosis is associated with its apoptotic activity (2). Alternative splicing produces the DIDO-1, -2 and -3 isoforms (also termed DIO-1, -2, -3), whose targeted disruption in mice produces a phenotype similar to myelodysplastic/myeloproliferative disease (MPS/MPD) in humans (3). DIDO3, the largest of the splice variants, is associated with the centrosome and plays a role in mitotic checkpoint and chromosome stability (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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